The Effectiveness and Mechanism of Toona sinensis Extract Inhibit Attachment of Pandemic Influenza A (H1N1) Virus
Figure 4
Effect of TSL-1 against pandemic influenza A (H1N1) infection was demonstrated by attachment assay and penetration assay. Effect of TSL-1 with the treatment procedures of attachment (a) or penetration (b) on influenza viral RNA synthesis in infected cells was analyzed by quantitative RT-PCR. Cell culture supernatants were collected and examined from the treatment procedures of attachment (c) or penetration (d) for virus yield by measured NA activity. Virus yield expressed as percent NA activity of culture supernatants of drug-free but only virus infected cells. Inhibitory effects of TSL-1 with the treatment procedures of attachment (e) or penetration (f) on virus adsorption to guinea pig red blood cells. Data were the mean ± SEM calculated from three independent experiments in triplicate. Significance compared with vehicle control, *.
