The Effectiveness and Mechanism of Toona sinensis Extract Inhibit Attachment of Pandemic Influenza A (H1N1) Virus
Figure 7
Effects of TSL-1 on the protein levels of VCAM-1, ICAM-1, E-selectin, fractalkine, IL-8, Bcl2-A1, and TLR-3 in pandemic influenza A (H1N1) virus-infected A549 cells. (a) After culturing A549 cells with/without the treatment of 25 μg/mL TSL-1 or 10 μg/mL Amantadine in the presence or absence of H1N1 viral infection, cell extracts were subjected to 12% SDS-PAGE and Western blot analysis with the respective primary antibody against VCAM-1, ICAM-1, E-selectin, fractalkine, IL-8, Bcl2-A1, and TLR-3. Tubulin protein was used as an internal control. Densitometric analyses of (b) VCAM-1, ICAM-1, E-selectin, fractalkine, IL-8, (c) Bcl2-A1, and TLR-3 were quantified and presented as mean values ± SEM of three similar independent experiments. Significance compared with vehicle control, *.
