Figure 5: Angelica root extract and DBT stimulate transcriptional activity of estrogen-responsive element of MCF-7 cells. (a) Three repeats of estrogen responsive elements (ERE: 5′-GGT CAC AGT GAC C-3′) were subcloned into a luciferase-reporter vector called pERE-Luc (upper panel). This reporter was stably transfected to MCF-7 cells, which were treated with Angelica root extracts at 0.5 mg/mL for 48 hours. 17-β-estradiol (100 nM) was used as a positive control. Values are expressed in percentage of increase as compared with control cultures (without herbal extract) and are in mean ± SD, where , each with triplicate samples. . (b) Cultures were treated as in (a) with different DBT at 0.7 mg/mL. Values are expressed in the fold of change as compared with control cultures (without herbal extract) and are in mean ± SD, where , each with triplicate samples. .