Methanol Extract of Artemisia apiacea Hance Attenuates the Expression of Inflammatory Mediators via NF-κB Inactivation
Figure 3
Effect of MEAH on the expression of proinflammatory enzymes in LPS-stimulated Raw264.7 cells. The cells were pretreated with 30 or 100 μg/mL MEAH for 1 h and then continuously exposed to 1 μg/mL LPS for 18 h. iNOS and COX-2 were immunoblotted in the cell lysates. Equal proteins loading among the samples was verified by actin immunoblotting (a). The relative iNOS and COX-2 protein levels were assessed by scanning densitometry (b, c). Data represent mean ± S.D. of three separated experiments (significant as compared with vehicle-treated control cells, **; significant as compared with LPS-treated cells, ##; the expression level of iNOS and COX-2 in control cells = 1).
