Anthocyanins from Vitis coignetiae Pulliat Inhibit Cancer Invasion and Epithelial-Mesenchymal Transition, but These Effects Can Be Attenuated by Tumor Necrosis Factor in Human Uterine Cervical Cancer HeLa Cells
Figure 1
Dose-dependent inhibitory effects of AIMs on HeLa cell proliferation and invasion. (a) Growth inhibition of HeLa cells. Cells were seeded at 5 × 104 cells/mL and treated with AIMs for 24 h with the indicated concentrations. (b) Effects on invasion of HeLa cells. The cells (5 × 104 cells) were loaded on precoated Matrigel 24-well invasion chambers (BD Biosciences) in the presence or absence of AIMs (25–400 μg/mL). HeLa cells were treated with AIMs for 18 hr in a Matrigel-coated transwell. (c) MMP-2 and MMP-9 protein levels were measured by gelatin zymography. Cells were incubated for 48 h without or with AIMs (25–400 μg/mL). Values represent means ± SD from three independent experiments. * versus control.
