Anthocyanins from Vitis coignetiae Pulliat Inhibit Cancer Invasion and Epithelial-Mesenchymal Transition, but These Effects Can Be Attenuated by Tumor Necrosis Factor in Human Uterine Cervical Cancer HeLa Cells
Figure 4
Effects of AIMs on NF-κB-regulated proteins involved in cancer metastasis. (a) Cells (5 × 104 cells) were either left untreated or pretreated with AIMs (25–400 μg/mL) for 24 h. (b)–(d) Cells (5 × 104 cells) were either left untreated or pretreated with AIMs (400 μg/mL) for 24 h and then were exposed to TNF (10 nM) for indicated times. Whole-cell extracts were prepared, and 30 μg of the whole-cell lysate was analyzed by Western blot using antibodies against various NF-κB-regulated proteins involved in (b) the cancer cell proliferation, (c) anti-apoptosis, and (d) invasion (MMP-2 and MMP-9), adhesion (ICAM-1), and angiogenesis (VEGF). Data were representative of two independent experiments.
