6-Shogaol Protects against Oxidized LDL-Induced Endothelial Injruries by Inhibiting Oxidized LDL-Evoked LOX-1 Signaling
Figure 3
Roles of the LOX-1 receptor in 6-shogaol-involved cell protection. Human umbilical vein endothelial cells (HUVECs) were exposed to 30 μM 6-shogaol, 200 μg/mL oxidized low-density lipoprotein (oxLDL), and a combination of 6-shogaol and oxLDL. LOX-1 full-length cDNA was overexpressed in HUVECs or LOX-1 siRNA was applied to HUVECs. LOX-1 full-length cDNA was overexpressed in HUVECs (a), and its effects on cell apoptosis were also analyzed by flow cytometry (b). Effects of LOX-1 siRNA on cell apoptosis were determined using flow cytometry (c). Each value represents the mean ± s.e.m. for . The symbols * and #, respectively, indicate that the values significantly differ from control and oxLDL-treated groups, . The symbol Δ means that the value significantly differs from the 6-shogaol + oxLDL-treated group, .
