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Evidence-Based Complementary and Alternative Medicine
Volume 2013 (2013), Article ID 516727, 10 pages
http://dx.doi.org/10.1155/2013/516727
Research Article

Antioxidant Activity and Cardioprotective Effect of a Nonalcoholic Extract of Vaccinium meridionale Swartz during Ischemia-Reperfusion in Rats

1Laboratorio de Ciencia de Alimentos, Universidad Nacional de Colombia, Sede Medellín, Medellín, Colombia
2CONICET, Centro de Investigaciones Cardiovasculares, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Argentina
3Departament de Farmacologia, Facultat de Farmàcia, Universitat de València, Burjassot, 46100 Valencia, Spain
4Cátedra de Farmacología Básica, Facultad de Ciencias Médicas, Universidad Nacional de La Plata and CIC Provincia de Buenos Aires, La Plata, Argentina

Received 30 May 2012; Revised 13 December 2012; Accepted 21 December 2012

Academic Editor: Adair Roberto Soares Santos

Copyright © 2013 Yasmin E. Lopera et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Our objective was to assess the antioxidant properties and the effects against the reperfusion injury of a nonalcoholic extract obtained by fermentation from the Colombian blueberry, mortiño (Vaccinium meridionale Swartz, Ericaceae). Antioxidant properties were assessed by in vitro systems. To examine the postischemic myocardial function, isolated rat hearts were treated 10 min before ischemia and during the first 10 min of reperfusion with the extract. To analyze the participation of nitric oxide (NO), other experiments were performed in the presence of nitric oxide synthase (NOS) inhibition with -nitro-L-arginine methyl ester (L-NAME). In cardiac tissue thiobarbituric acid reactive substances (TBARS) concentration, reduced glutathione (GSH) content, endothelial NOS (eNOS), and Akt expression were also measured. The blueberry extract showed higher total phenols and anthocyanins contents, scavenging activity of superoxide radical and systolic and diastolic function was improved, TBARS diminished, GSH was partially preserved, and both NOS and Akt expression increased in hearts treated with the extract. These beneficial effects were lost when eNOS was inhibited. In resume, these data show that the increase of eNOS expression via Akt and the scavenging activity contribute to the cardioprotection afforded by acute treatment with Colombian blueberry extract against ischemia and reperfusion injury.