The Aqueous Extract of Rhizome of Gastrodia elata Protected Drosophila and PC12 Cells against Beta-Amyloid-Induced Neurotoxicity

Figure 5

Antioxidative effect of GE on Aβ-induced cytotoxicity in PC12 cells. (a) GE extract reduced Aβ-induced oxidative stress in flow cytometric analysis of DCF positive cells. The fluorescence intensity of DCF was measured after PC12 cells were exposed to 20 μM Aβ for 48 h, followed by 20 μM H2DCF-DA for 15 min. 48 h treatment of GE extract increased the activities of antioxidative enzymes (b) superoxide dismutase (c) catalase and (d) glutathione peroxidase in 20 μM Aβ-treated cells. Results are the means ± SD from three separate experiments. ^#𝑃<0.05, ^##𝑃<0.01 relative to control; *𝑃<0.05, **𝑃<0.01, ***𝑃<0.001 relative to Aβ treatment only by one-way ANOVA.

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