Potential Therapeutic Role of Hispidulin in Gastric Cancer through Induction of Apoptosis via NAG-1 Signaling
Figure 2
Hispidulin can induce cancer apoptosis by caspase-3 activation. (a) Cell apoptosis assay after treatment by flow cytometry apoptosis analysis. (b) Caspase-3 activity assay after treatment in various dosage. (c) Caspase-3 activity assay 6, 12, 24, and 48 h after 50 μM Hispidulin treatment. (d) The Western blot analysis for apoptosis related protein analysis after Hispidulin treatment. Expression of -actin was used as an internal control. (e) The Western blot analysis for apoptosis related protein analysis 6, 12, 24, and 48 h after treatment. Expression of -actin was used as an internal control. Each column represents the mean ± SD (; ; ).