Research Article

Greater Skeletal Gains in Ovary Intact Rats at Maturity Are Achieved by Supplementing a Standardized Extract of Butea monosperma Stem Bark that Confers Better Bone Conserving Effect following Ovariectomy and Concurrent Treatment Withdrawal

Figure 1

Experimental design. Three-week-old (weaned) female SD rats were given either vehicle (daily/p.o.) or ASF (100 mg/kg/d/p.o.) for 12 weeks (baseline). At baseline, BMD and microarchitectural parameters were recorded and serum TAS was measured. Rats were then either sham operated or OVx and were given either vehicle or E2 (10 μg/kg/d/p.o.) for another 12 weeks (endpoint). BMD and microarchitectural parameters were recorded at 4- and 8-week following OVx. After 12-week following OVx (endpoint), all animals were killed; blood, urine (24 h), tibia, femur, and vertebrae were harvested and analyzed.
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