Greater Skeletal Gains in Ovary Intact Rats at Maturity Are Achieved by Supplementing a Standardized Extract of Butea monosperma Stem Bark that Confers Better Bone Conserving Effect following Ovariectomy and Concurrent Treatment Withdrawal
Table 4
Effect on bone formation indices, microarchitecture, and strength at femur diaphysis following ovariectomy and treatment withdrawal at the endpoint.
Parameters
Sham + vehicle (1% gum acacia)
OVx + vehicle (1% gum acacia)
OVx + vehicle (1% gum acacia) (ASFW)
OVx + E2 (10 μg/kg/day)
Bone formation indices at femur mid-diaphysis
pMS/BS (%)
96.96 ± 0.5p
85.92 ± 2.72
95.43 ± 0.46q
89.87 ± 1.86c,g
pMAR (μm/day)
0.43 ± 0.01p
0.2 ± 0.01
0.34 ± 0.01p,a
0.23 ± 0.001a,e
pBFR /BS (μm3/μm2/yr)
3.79 ± 0.05p
1.87 ± 0.08
3.13 ± 0.13p,b
2.05 ± 0.16a,e
Micro-CT measurements at femur mid-diaphysis
B.Ar (mm2)
4.7 ± 0.14q
3.79 ± 0.06
4.53 ± 0.06q
4.62 ± 0.21q
Ct.Th (mm)
0.49 ± 0.01r
0.35 ± 0.02
0.47 ± 0.02r
0.4 ± 0.03
T.Ar (mm2)
8.16 ± 0.05
8.19 ± 0.21
8.13 ± 0.1
8.25 ± 0.6
Ma.Ar (mm2)
3.37 ± 0.06r
4.66 ± 0.26
3.45 ± 0.13q
3.77 ± 0.32r
MMI (mm4)
7.33 ± 0.61r
10.2 ± 0.69
7.63 ± 0.37r
8.41 ± 0.65
Bone strength at femur mid-diaphysis
Ultimate load (N)
142.66 ± 10.5p
101.33 ± 4.27
161.66 ± 5.49p
149.75 ± 1.88q
Energy (mJ)
70.66 ± 2.45q
42.65 ± 3.85
82.91 ± 6.52q
65.241 ± 1.16r
Stiffness (N/mm)
247.62 ± 13.39p
133.58 ± 20.91
252.475 ± 13.12p
248.161 ± 22.41q
Values represent mean ± SEM; rats/group. p, q, and r compared to OVx + vehicle; a, b, and c compared to sham; e and g compared to ASFW. pMS/BS: periosteal mineralizing surface per bone surface, pMAR: periosteal mineral apposition rate, pBFR/BS: periosteal bone formation rate/bone surface. B.Ar: bone area, Ct.Th.: cortical thickness, T.Ar: periosteal area, MMI: mean polar moment of inertia, Ma.Ar: medullary area.