Curcumin Protects against 1-Methyl-4-phenylpyridinium Ion- and Lipopolysaccharide-Induced Cytotoxicities in the Mouse Mesencephalic Astrocyte via Inhibiting the Cytochrome P450 2E1

Figure 4

Cur as well as DAS ameliorated cell viability of the primary mesencephalic astrocytes induced by MPP⁺ and LPS. (a) The H₂O₂ production of the primary cultured mesencephalic astrocytes. (b) The GSH-Px activity of the primary cultured mesencephalic astrocytes. The primary cultured mesencephalic astrocytes were nonpretreated or pretreated with vehicle (DMSO) and Cur (3 μM) for 30 min and then incubated with PBS, MPP⁺, or LPS for 48 h. The cultured media were measured for H₂O₂ production with H₂O₂ assay kit. And the lysates determined GSH-Px activity using GSH-Px assay kit. (c) Effect of Cur and DAS on the cell viability of the primary cultured mesencephalic astrocytes induced by MPP⁺ and LPS. The primary cultured mesencephalic astrocytes were nonpretreated or pretreated with vehicle (DMSO), Cur (3 μM), and DAS (500 μM) and then incubated with PBS, MPP⁺, LPS, or EtOH for 48 h. EtOH was as a positive CYP2E1 inducer. Data are presented as mean ± SD of three independent experiments, ^∗𝑃<0.05,^∗∗𝑃<0.01, and  ^∗∗∗𝑃<0.001 compared to the control group; ^#𝑃<0.05 and  ^##𝑃<0.01 compared to the MPP⁺ or LPS treatment alone group.

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