Resveratrol Downregulates Interleukin-6-Stimulated Sonic Hedgehog Signaling in Human Acute Myeloid Leukemia

Table 1

Primer sequences used for RT-PCR analysis.

Genes	Sequences	Annealing temp.^a	Products (bp)
Shh:		33.8	477
Forward	5′-CGCACGGGGACAGCTCGGAAGT-3′
Reverse	5′-CTGCGCGGCCCTCGTAGTGC-3′
Gli-1:		49	185
Forward	5′-TTCCTACCAGAGTCCCAAGT-3′
Reverse	5′-CCCTATGTGAAGCCCTATTT-3′
GAPDH:		55	574
Forward	5′-CCACCCATGGCAAATTCCATGGCT-3′
Reverse	5′-TCTAGACGGCAGGTCAGGTCCACC-3′

aThe PCR thermal cycle profile consisted of 1 cycle of denaturation for 5 min at 95°C; 30 cycles of denaturation for 30 sec at 95°C, annealing of primers for 45 sec at different temperatures as shown in this Table, and extension for 50 sec at 72°C; and 1 cycle of a final extension step at 72°C for 10 min.