Triterpenoid Saponins Isolated from Platycodon grandiflorum Inhibit Hepatitis C Virus Replication

Figure 4

Purification of HCV NS5B protein. (a) HCV NS5B protein (1 μg) eluted from glutathione sepharose affinity column was subjected to SDS-polyacrylamide gel electrophoresis and visualized by Coomassie Brilliant Blue R-250; (b) NS5B protein purified from figure legend to A was immunoblotted with an anti-NS5B monoclonal antibody and visualized with the enhanced chemiluminescence detection. The arrowhead indicates the NS5B protein.