Electroacupuncture at Zusanli (ST36) Prevents Intestinal Barrier and Remote Organ Dysfunction following Gut Ischemia through Activating the Cholinergic Anti-Inflammatory-Dependent Mechanism
Figure 5
Electroacupuncture at ST36 prevents the decrease of intestinal ZO-1 protein expression. Intestinal extracts were obtained from animals 60 min following reperfusion for measurement of ZO-1 protein expression using Western blot. Representative Western blot for the ZO-1 protein is shown with its corresponding GAPDH loading control to demonstrate equal protein load in all lanes. EA ST36, VNS, and PNU282987 resulted in preservation of protein expression when performed on animals with an intact neuroenteric axis. Significant reduction in ZO-1 expression was seen after iI/R treated with SEA, and if abdominal vagotomy or α-BGT was performed before EA ST36. # versus IR group, ( animals per group).
