The Preventive Effect of Biochanin A on Bone Loss in Ovariectomized Rats: Involvement in Regulation of Growth and Activity of Osteoblasts and Osteoclasts

Figure 2

Effects of BCA and E2 on RANKL and OPG mRNA expression. The mRNA levels for RANKL (b) and OPG (c) in the distal femur were determined by RT-PCR. Expression was normalized to that of GAPDH and expressed as a percentage of that in the sham group. The bars represent the mean ±SD for ten samples. *𝑃<0.05 compared with the sham group; ^#𝑃<0.05 compared with the OVX C group; ^∇𝑃<0.05 compared with the E2 group. RANKL: receptor activator of nuclear factor-κB ligand; OPG: osteoprotegerin; C: control; BCA: biochanin A; E2: 17β-estradiol; OVX: ovariectomized.

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