Interaction of Natural Dietary and Herbal Anionic Compounds and Flavonoids with Human Organic Anion Transporters 1 (SLC22A6), 3 (SLC22A8), and 4 (SLC22A11)

Figure 3

Dose-response effects for 1,3-dicaffeoylquinic acid and 18β-glycyrrhetinic acid on hOAT1-mediated PAH transport. One-minute uptake of [³H]PAH (1 μM) in CHO-hOAT1 cells was measured in the presence of increasing concentrations (10⁻⁷ to 5×10⁻⁴  M) of test compounds. Data were corrected by subtracting background PAH accumulation measured in empty vector transfected cells. IC₅₀ values were determined with nonlinear regression and the “log(inhibitor) versus response” model using GraphPad Prism software. Experiments were repeated three times with triplicate samples and graphs shown are from representative experiments with values plotted as mean±S.D. (𝑛=3). The data were used to generate mean IC₅₀±S.E.M. estimates.

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