Bees’ Honey Attenuation of Metanil-Yellow-Induced Hepatotoxicity in Rats

Figure 1

Binding activity of NF-κB-p65 (a). Group 1, untreated (normal control); groups 2–4, rats treated with 50, 100, 200 mg/kg metanil yellow, respectively; and groups 5–7, rats were treated with metanil yellow as in groups 2–4 plus bees’ honey 2.5 mg·kg⁻¹d⁻¹ for 8 weeks. Binding activity of NF-κB-p65 to its consensus sequence was assayed by EMSA of total protein extracts. Quantification of activated NF-κB-p65 was performed by densitometric analysis of relative EMSA band intensities (b) using the normal control band as a reference band. All values expressed as mean ± S.D. of triplicate tests (𝑛=10). ^a𝑃<0.05 versus group1, ^b𝑃<0.05 versus group 2, ^c𝑃<0.05 versus group 3, ^d𝑃<0.05 versus group 4.

(a)

(b)