Bees’ Honey Attenuation of Metanil-Yellow-Induced Hepatotoxicity in Rats

Table 2

Antioxidant parameters as well as AGE levels of rat liver homogenate. Group 1, control group; groups 2–4, rats treated with 50, 100, and 200 mg metanil yellow/kg body weight for 8 weeks, respectively; groups 5–7, rat groups treated with 50, 100, and 200 mg metanil yellow/kg body weight plus 2.5 g/kg bees’ honey daily for 8 weeks, respectively.

	Group 1	Group 2	Group 3	Group 4	Group 5	Group 6	Group 7
MDA, nmol/mg protein	0.42 ± 0.06	1.49 ± 0.19^a	5.1 ± 0.85^a	9.37 ± 1.8^a	0.85 ± 0.02^b	1.45 ± 0.35^a,c	1.9 ± 0.92^a,d
GSH, nmol/mg protein	101 ± 5.8	72.5 ± 1.6^a	45.9 ± 4.6^a	26.7 ± 3.8^a	79.5 ± 2.1	78.5 ± 2.1^a,c	80.2 ± 1.3^a,d
GST, nmol/mg protein	18.6 ± 1.5	11.1 ± 1.3^a	6.29 ± 0.39^a	4.2 ± 0.94^a	14.76 ± 0.86^b	10.54 ± 0.45^a,c	10.3 ± 0.87^a,d
SOD, U/mg protein	15.8 ± 1.1	10.5 ± 2.5^a	9.6 ± 2.1^a	3.1 ± 1.2^a	14.25 ± 1.1^b	11.2 ± 1.3^a,c	10.97 ± 1.5^a,d
CAT, U/mg protein	5.95 ± 0.5	3.12 ± 0.2^a	2.41 ± 0.31^a	1.1 ± 0.11^a	4.56 ± 0.54^b	4.29 ± 0.23^c	3.1 ±0.67^a,d
GSH-Px, U/mg protein	4.95 ± 0.21	3.1 ± 0.31^a	2.05 ± 0.12^a	1.15 ± 0.10^a	4.67 ± 0.65^b	4.12 ± 0.32^c	3.87 ± 0.56^d
AGE, ng/mg protein	20.6 ± 4.5	26.2 ± 5.5^a	36.5 ± 5.5^a	48.7 ± 8.5^a	22.2 ± 5.5^b	28.2 ± 5.5^a,c	32.2 ± 5.5^a,d

All values were expressed as mean ± S.D. of triplicate tests (𝑛 = 10).
^a𝑃 < 0.05 versus group 1, ^b𝑃 < 0.05 versus group 2, ^c𝑃 < 0.05 versus group 3, ^d𝑃 < 0.05 versus group 4.