Therapeutic Effects of Water Soluble Danshen Extracts on Atherosclerosis
Figure 2
Anti-inflammatory and antiapoptotic effects of Danshen extract on human umbilical vein endothelial cells. (a) Western blotting (left panel) and immunohistochemical staining (right panel) for VCAM-1. Human umbilical vein endothelial cells (HUVECs) were pretreated with Danshen extract (DE) (100 μg/mL) 24 h prior to exposure with TNF-α (10 ng/mL) for 24 h. Representative immunohistologic sections (200X) of VCAM-1 in balloon-injured rat carotid arteries were shown. (b)-(c) Western blotting for phosphorylated JNK and NF-κB p65. HUVECs were treated with TNF-α (100 ng/mL) for 15 m with or without pretreatment with DE (100 μg/mL) for 24 h. (d) Western blotting for hemoxygenase-1 in both HUVECs with or without treatment of TNF-α (100 ng/mL) for 15 min. DE: Danshen extract.
