Garlic-Derived S-Allylmercaptocysteine Ameliorates Nonalcoholic Fatty Liver Disease in a Rat Model through Inhibition of Apoptosis and Enhancing Autophagy

Figure 6

Cotreatment with SAMC further enhanced hepatic autophagy through inhibition of mTOR activity. Protein expressions of (a) phosphorylated and total mTOR, (b) vps34, (c) beclin 1, (d) Atg 12, (e) LC3 II and (f) p62 were measured by Western blot and then quantified by ImageJ software. Data presented are expressed as mean ± SEM (𝑛=7), and experimental groups marked by different letters represented significant differences between groups at 𝑃<0.05 (Kruskal-Wallis test followed by Dunn’s post hoc test). N + S: NAFLD + SAMC cotreatment.

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