Isolation of Resveratrol from Vitis Viniferae Caulis and Its Potent Inhibition of Human Tyrosinase

Figure 3

Purification of compound X and its identification as resveratrol. The EtOAc fraction derived from VVC extract was separated on a Sephadex LH20 column (a) And the elution fractions were analyzed by TLC for compound X. Compound-X-containing fractions were then pooled, concentrated, and subjected to second chromatography on an ODS-A column (b). Elution fractions containing compound X were combined and evaporated to obtain purified compound X, which was compared with commercially sourced resveratrol by TLC (c). Ascending TLC was run on a silica-coated plate using a mixture of chloroform, methanol, and acetic acid (90 : 10 : 1, v/v/v) as the mobile phase.