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Evidence-Based Complementary and Alternative Medicine
Volume 2013 (2013), Article ID 659391, 7 pages
http://dx.doi.org/10.1155/2013/659391
Research Article

Antidepressive-Like Property of Dichloromethane Fraction of Pimenta pseudocaryophyllus and Relevance of Monoamine Metabolic Enzymes

1Department of Physiological Sciences, Federal University of Goiás, Campus Samambaia, 74001-970 Goiâania, GO, Brazil
2Laboratory of Neuropharmacology, Department of Pharmacology, CEP 88040-970 BLOC D CCB, Federal University of Santa Catarina, UFSC, Florianópolis, SC, Brazil
3Sciences and Technology Unit, Goias State University, BR 153, No. 3105, CEP 75132-903, Fazenda Barreiro do Meio, Anápolis, GO, Brazil
4Faculty of Pharmacy, Federal University of Goiás, Setor Universitário, CEP 74001-970 Goiâania, GO, Brazil
5Department of Physiological Sciences, Institute of Biology, Federal Rural University of Rio de Janeiro, BR465, km 07, Seropédica, 23890-000 Rio de Janeiro, RJ, Brazil

Received 29 September 2012; Revised 6 December 2012; Accepted 6 December 2012

Academic Editor: David Mischoulon

Copyright © 2013 James Oluwagbamigbe Fajemiroye et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Pimenta pseudocaryophyllus popularly referred to as craveiro is considered as a calming agent in different local preparations. The present study attempted to examine antidepressant-like effect of dichloromethane fraction (DF) and role of monoamine oxidase (MAO), tryptophan, and tyrosine hydroxylase. Based on the research focus, tail suspension (TS), forced swimming (FS), and open field (OF) tests were conducted after oral administration of DF (125, 250, or 500 mg/Kg). Ex vivo assay of MAO was also conducted to evaluate inhibitory effect of DF (250 mg/Kg). Administration of DF elicits antidepressant-like response in the TS and FS. However, DF 500 mg/Kg did not alter mice performance in these models. The data obtained in the OF showed a reduction in total crossing and rearing activity; these effects suggest motor interference in TS and FS performance. Mice pretreatment with p-chlorophenylalanine methyl ester (PCPA) (100 mg/kg, i.p.—serotonin biosynthesis inhibitor) for 4 consecutive days or acute administration of α-methyl-p-tyrosine (AMPT) (100 mg/kg, i.p.—catecholamine synthesis inhibitor) blocked anti-immobility effect of DF in the FS. In ex vivo assay of MAO, DF did not inhibit catabolic activity of MAO. Our findings support antidepressant-like activity of DF and suggest an effect that depends on monoamine biosynthesis.