Inhibition of Helicobacter pylori CagA-Induced Pathogenesis by Methylantcinate B from Antrodia camphorata
Figure 2
Translocation of CagA was attenuated by MAB. AGS cells were treated or untreated with MAB and infected with CagA-EGFP H. pylori at 37°C for 6 h. Cells were fixed and stained with DAPI (blue) to visualize bacteria and the cell nucleus. Samples were analyzed by confocal microscopy. The colocalization of GFP with DAPI appears cyan in the overlay to show bacteria (arrows). The translocated CagA-EGFP of green fluorescence signals (arrow heads) were quantified and normalized with DAPI signals. The data were analyzed using the Pearson correlation coefficient and presented in the right panel. Statistical significance was determined for 3 independent experiments. **; Bar, 10 μm.
