Antidiabetic Effect and Mode of Action of Cytopiloyne
Figure 2
Cytopiloyne-mediated insulin secretion depends on pancreatic β cells. (a) Rat pancreatic islets were incubated with KRB buffer containing vehicle, glimepiride (GLM, 0 to 10 μM), or cytopiloyne (CP, 1.5 to 42 μM) in the absence or presence of 16.7 mM glucose (HG). The insulin levels were determined using an insulin ELISA kit. The data are presented as mean ± SEM of 3 independent experiments. (b) Fed C57BL mice, which had already received an injection of STZ, were administered an oral dose of vehicle, cytopiloyne (CP, 0.1, 0.5, and 2.5 mg/kg), and an intraperitoneal injection of insulin (Ins, 2.5 IU/kg). Postprandial blood sugar levels in the STZ-treated mice were determined using a glucometer. (c) Fed C57BL mice, which had already received STZ, were orally administered a single dose of vehicle, cytopiloyne (CP, 0.5 and 2.5 mg/kg), glimepiride (GLM, 2.5 mg/kg), or metformin (Met, 60 mg/kg), followed by an intraperitoneal injection with insulin (Ins). Postprandial blood sugar levels in the STZ-treated mice were determined using a glucometer. Results are expressed as mean ± SEM from 3 independent experiments, and was considered to be statistically significant (*). The number of mice () is indicated in parentheses.
