686018.fig.003a
(a)
686018.fig.003b
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686018.fig.003c
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Figure 3: (a) Illustrative scattering diagram representing the percentage, distribution, and selection of B-cells populations in Flow cytometry of peritoneal washing fluid harvested from a control mouse. (A) lymphocyte and phagocyte gates identified on the basis of cell size ( -axis) and lumpiness ( -axis); (B) from the lymphocyte gate, a secondary diagram was extracted representing B1 (CD19+ CD23−) and B2 (CD19+ CD23+) cells; (C) from the phagocyte gate, another secondary diagram was extracted, representing B1 derived phagocytes—BDP (CD11b+ CD19+) and mature phagocytes—MP (CD11b+ CD19−). Graphs obtained from Flow Jo 8.7 software. (b) Illustrative scattering diagram representing the distribution and selection of B-cells populations in Flow cytometry of local lymph node cell suspension from a thymulin 5CH treated mouse. (A) lymphocyte gate identified after the cell size ( -axis) and lumpiness ( -axis); (B) from the lymphocyte gate, a secondary diagram was extracted representing B1 (CD19+ Cd11b+) cells; (C) from the B1 gate, another secondary diagram was extracted, representing B1a (CD11b+ CD5+) cells. Graphs obtained from Attune 1.2 software. (c) Illustrative scattering diagram representing the distribution and selection of T-cell subpopulations in Flow cytometry of lymph node cell suspension, from a thymulin 5CH treated mouse. The diagram was extracted from the lymphocyte gate. The predominant population was CD8+ cells (R14). Graphs obtained from Attune 1.2 software.