Song Bu Li Decoction, a Traditional Uyghur Medicine, Protects Cell Death by Regulation of Oxidative Stress and Differentiation in Cultured PC12 Cells
Figure 2
SBL prevents cell death in tBHP-treated PC12 cells. (a) Cultured PC12 cells were treated with tBHP (0–600 μM) for 3 hours to determine the cytotoxicity of tBHP by cell viability assay. (b) PC12 cells were pretreated with SBL (3–12 μg/mL), NRR (6 and 12 μg/mL) and volatile oil (2 μg/mL) for 24 hours before the addition of tBHP (150 μM) for 3 hours. The neuroprotective effect of SBL by cell viability assay was shown. Vitamin C (1 mM) served as a positive control. The viability of PC12 cells is shown in percentage of MTT value relative to normal control. Data are expressed as Mean ± SEM, where , each with triplicate samples. * and ** as compared to the group (with tBHP alone).
