Song Bu Li Decoction, a Traditional Uyghur Medicine, Protects Cell Death by Regulation of Oxidative Stress and Differentiation in Cultured PC12 Cells
Figure 4
SBL induces ARE transcriptional activity and detoxifying enzymes in PC12 cells. (a) Four repeats of antioxidant responsive element (ARE: 5′-TGACnnnGCA-3′) tagged with luciferase-reporter vector called pARE-luc (upper panel). This reporter was stably transfected to PC12 cells, which were treated with SBL, NRR, and volatile oil for 24 hours (lower panel). tBHQ (3 μM) was used as a positive control. (b) Cultured PC12 cells treated with SBL (12 μg/mL) and tBHQ (3 μM) for 24 hours. Total RNAs were isolated from cultured PC12 cells and then reversed transcribed into cDNAs for the detection of mRNAs encoding for GST, GCLC, GCLM, and NQO1 by real-time PCR analysis. The GAPDH served as internal control. Values are expressed as the fold of increase to basal reading (untreated culture), and in Mean ± SEM, where , each with triplicate samples. * and **.
