689865.fig.002a
(a)
689865.fig.002b
(b)
689865.fig.002c
(c)
689865.fig.002d
(d)
689865.fig.002e
(e)
689865.fig.002f
(f)
Figure 2: Effects of girinimbine on the morphological changes of A549 cells after 24 h treatment. Apoptosis was monitored by phase contrast ((a) control, (b) treatment 9 μM, and (c) treatment 19 μM) and fluorescence microscopy ((d) control, (e) treatment 9 μM, and (f) treatment 19 μM). Arrows indicate the cell shrinkage, ruffling, and blebbing of cell membrane while arrowheads indicate the annexin V binding to phosphatidylserine. Magnification 20X.