Evidence-Based Complementary and Alternative Medicine

Research Article

Renal Protective Role of Xiexin Decoction with Multiple Active Ingredients Involves Inhibition of Inflammation through Downregulation of the Nuclear Factor-B Pathway in Diabetic Rats

Table 2

Nucleotide sequence of primers used in real-time PCR.
Gene PrimersNucleotide sequence 5′-3′
ICAM-1Forward;
reverse		AGGTATCCATCCATCCCAC;
GCCGAGGTTCTCGTCTTC
MCP-1Forward;
reverse		TCTCTTCCTCCACCACTATGCA;
GGCTGAGACAGCACGTGGAT
TGF-β1Forward;
reverse		GCTAATGGTGGACCGCAACAAC;
TCTGGCACTGCTTCCCGAATG
NF-κBp65Forward;
reverse		GGCAGCACTCCTTATCAA;
GGTGTCGTCCCATCGTAG
β-actinForward;
reverse		TTATCGGCAATGAGCGGTTC;
AGCACTGTGTTGGCATAGAG
ICAM-1: intercellular adhesion molecule-1; MCP-1: monocyte chemotactic protein-1; TGF- 1: transforming growth factor- 1; NF- Bp65: nuclear factor Bp65.