Inhibition of NADPH Oxidase Mediates Protective Effect of Cardiotonic Pills against Rat Heart Ischemia/Reperfusion Injury
Figure 2
The effects of CP pretreatment on I/R-induced apoptosis and the expression of apoptosis related proteins. (a) Representative images of TUNEL stained myocardial sections in various groups. Apoptotic nuclei are indicated by TUNEL staining (green), and total nuclei are identified by Hoechst staining (blue). Arrowheads indicate normal nuclei. Arrows indicate TUNEL positive cells. . (b) Representative photographs of immunohistochemistry staining for Caspase-3 in different groups. (c) Representative images of myocardial slices stained for Bax in different groups. (d) Representative slices of immunohistochemistry staining for Bcl-2 protein in various groups. Immunohistochemistry positive staining cells are shown in brown color. . (e) to (h) Shown are statistical analysis of TUNEL positive nuclei, Caspase-3 expression, Bax expression, and Bcl-2 expression, respectively, in five different groups. 1, 2, 3, 4, and 5 which denotes Sham group, I/R group, CP group, CP group, and CP group, respectively. Data are presented as mean ± SEM (). versus Sham group, versus I/R group.
