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Evidence-Based Complementary and Alternative Medicine
Volume 2013 (2013), Article ID 748687, 9 pages
http://dx.doi.org/10.1155/2013/748687
Research Article

Antiresorptive Activity of Bacillus-Fermented Antler Extracts: Inhibition of Osteoclast Differentiation

1Laboratory of Translational Therapeutics, Pharmacology Research Center, Bio-Organic Science Division, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseong-gu, Daejeon 305-600, Republic of Korea
2Food Functional Research Division, Korean Food Research Institute, Sungnam 463-746, Republic of Korea
3Department of Pharmacy, Sunchon National University, Suncheon 540-742, Republic of Korea
4Department of Histology, College of Veterinary Medicine, Kyungpook National University, Sankyuk-dong, Buk-gu, Daegu 702-701, Republic of Korea
5Korean Medicine Biofermentation Co. Ltd., Seoul 137-894, Republic of Korea

Received 4 July 2012; Revised 3 December 2012; Accepted 3 December 2012

Academic Editor: HuanBiao Mo

Copyright © 2013 Sik-Won Choi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Antlers have been traditionally used for thousands of years as a natural product with medicinal and pharmaceutical properties. In developing healthy foods, Bacillus-mediated fermentation is widely used to enhance the biological activity of nutrients in foods. Recently, fermentation was shown to enhance the osteogenic activity of antlers. This study aimed to elucidate the antiresorptive activity of Bacillus-fermented antler and its mode of action. We found that Bacillus-fermented antler extract strongly inhibited osteoclast differentiation by downregulating the expression and activity of nuclear factor of activated T cells, cytoplasmic 1 (NFATc1). This extract also inhibited the activation of phospholipase Cγ2 (PLCγ2), a signaling molecule that could regulate NFATc1 transcriptional activity. This suggested that Bacillus-fermented antler extract could inhibit PLCγ2-NFATc1 signaling required for bone resorption and cell fusion. Consequently, Bacillus-fermented antler extract might benefit osteoclast-related disorders, including osteoporosis; furthermore, it may improve gastrointestinal activity.