Antiresorptive Activity of Bacillus-Fermented Antler Extracts: Inhibition of Osteoclast Differentiation
Figure 1
Fermented antler extract inhibits RANKL-induced osteoclast differentiation. (a) BMM cells were cultured for 4 days in the presence of RANKL (5 ng/mL) and M-CSF (30 ng/mL) with antler or fermented antler extract. Osteoclasts were visualized by TRAP staining. (b) The number of TRAP-positive osteoclasts (TRAP+OCs) was counted. (versus “the control”). (c) TRAP activity was measured. (versus “the negative control”); (versus “the group treated with RANKL alone”). (d) The effect of fermented antler extract on the viability of BMMs was evaluated by CCK-8 assay. Each experiment was performed in triplicate. Statistical differences were analyzed with the Student’s t-test and all quantitative values were presented as mean ± SD.
