Antiresorptive Activity of Bacillus-Fermented Antler Extracts: Inhibition of Osteoclast Differentiation
Figure 3
Fermented antler extract suppresses NFATc1-induced osteoclast differentiation. (a) BMMs were infected with pMX-IRES-GFP (GFP) or pMX-IRES-CA-NFATc1-GFP (CA-NFATc1-GFP) for 8 hrs with polybrene (10 μg/mL). The infected BMMs were cultured with M-CSF (30 ng/mL) and RANKL (5 ng) for 4 days in the presence or absence of fermented antler extract (25 μg/mL). After 4 days, cells were fixed, and the GFP expression was visualized under a fluorescence microscope. (b) BMMs were infected with GFP or CA-NFATc1-GFP and then cultured as described in (a). After 4-day culture, mature TRAP-positive osteoclasts were visualized by TRAP staining. (c) TRAP-positive cells (TRAP+OCs) were counted as osteoclasts. (versus “the GFP control”); ; (versus “the fermented antler-nontreated group”). (d) TRAP activity was measured at 405 nm. (versus “the GFP control”); ; (versus “the fermented antler-nontreated group”). Each experiment was performed in triplicate. Statistical differences were analyzed with the Student’s t-test and all quantitative values were presented as mean ± SD.
