Chondroprotective Activity of Murraya exotica through Inhibiting β-Catenin Signaling Pathway
Figure 4
(a) Changes in β-catenin, COX-2, and caspase-3 mRNA expression in the control and model groups and in the groups treated for 24 h with 50 mg/kg, 100 mg/kg, and 200 mg/kg M. exotica. qRT-PCR was used to detect changes in mRNA expression of these genes. GAPDH was used as internal control. These data were representative of results obtained from the analysis of three independent experiments. Data were presented by mean ± standard deviation of 4 replicates. as compared with model. (b) Chondrocytes were transfected with Fopflash or Topflash luciferase reporters. Transfected cultures were treated with 50 mg/kg, 100 mg/kg, and 200 mg/kg M. exotica for 24 h. Data were ratios of firefly luciferase units from the respective reporters to constitutive CMV-regulated Renilla luciferase units normalized to their respective model group cultures. Data were presented by mean ± standard deviation of 4 replicates. as compared with control.
