The Extract of Litsea japonica Reduced the Development of Diabetic Nephropathy via the Inhibition of Advanced Glycation End Products Accumulation in db/db Mice

Figure 4

Effect of LJE on nephrin expression in the renal glomeruli. (a) Immunohistochemical signal for nephrin. (b) In situ hybridization of nephrin mRNA. ×400 magnification. (c) Quantitative analysis of the nephrin protein signals in histological sections. (d) RT-PCR using a nephrin-specific primer. All data are expressed as the mean ± SEM (𝑛=8). ^∗𝑃<0.05 versus the NOR group, ^∗∗𝑃<0.01 versus the NOR group, ^#𝑃<0.05 versus the DM group, ^##𝑃<0.01 versus the DM group.

(a)

(b)

(c)

(d)