Achyrocline satureioides (Lam.) D.C. Hydroalcoholic Extract Inhibits Neutrophil Functions Related to Innate Host Defense

Figure 5

Effects of A. satureoides hydroalcoholic extract on in vivo leukocyte migration induced by LPS, LTB-4, and CINC-1 secretion. Air pouch animals received orally (1) sham (air pouch induced without treatment), (2) vehicle (PBS/ethanol 10%), (3) indomethacin (30 mg/kg), or (4) A. satureoides (100 mg/kg). After 1 h, LPSs from E. coli 026:B6 (1 mg/2 mL) or PBS were injected directly into the air pouch, and 1 h later, the number of cells in the pouch was quantified. (a) Number of neutrophils in the air pouch, (b) levels of LTB-4 on air pouch exudate, and (c) levels of CINC-1 secretion on air pouch exudate. Data are expressed as mean ± S.E.M. of 5-6 animals in each group. Statistical analysis was performed using ANOVA followed by Tukey’s test. ^∗𝑃<0.05 and ^∗∗∗𝑃<0.001 versus sham; ^#𝑃<0.05, ^##𝑃<0.01, and ^###𝑃<0.01 versus vehicle.

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