Antioxidant Effects of Quercetin and Naringenin Are Associated with Impaired Neutrophil Microbicidal Activity
Figure 5
Effects of naringenin and quercetin on the kinetics of the killing of S. aureus by neutrophils. Neutrophils ( cells/mL per assay) were incubated with opsonized bacteria ( cells/mL). Microbicidal activity was monitored in the presence and absence of 25 μM (▲) and 50 μM (▼) naringenin and 25 μM (♦) and 50 μM (+) quercetin. Killing activity was determined by aseptically removing the samples at intervals of 0, 30, 60, 90, and 120 min. The number of viable bacteria was evaluated by spread-plating suitable diluted samples on nutrient agar. The controls included bacteria alone (■) and bacteria plus neutrophils (●). The data are expressed as the mean ± SEM of three experiments.
