Subtoxic Levels of Apigenin Inhibit Expression and Secretion of VEGF by Uveal Melanoma Cells via Suppression of ERK1/2 and PI3K/Akt Pathways
Figure 4
RT-PCR analysis for the effects of apigenin on the expression of VEGF mRNA in uveal melanoma cells. Representative RT-PCR profiles from three experiments showed the mRNA expressions of VEGF by uveal melanoma cells (SP6.5 cell line) exposed to apigenin at 0, 1, 2, and 5 μM (a). After 6 h of culture with or without apigenin, cells were collected, mRNA was extracted, and RT-PCR analysis was performed as described in the text. GAPDH was used as an internal loading control. Apigenin inhibited the expression of VEGF mRNA in uveal melanoma cells in a dose-dependent manner (b).
