Effectiveness of Panax ginseng on Acute Myocardial Ischemia Reperfusion Injury Was Abolished by Flutamide via Endogenous Testosterone-Mediated Akt Pathway
Figure 6
Effects of RSE and flutamide on time course changes of activation and expression of PI3K/Akt/eNOS signaling. PI3K/Akt/eNOS signaling in heart was investigated by Western blot at six time points. The blot was reprobed sequentially with antibodies special for PI3K or phospho-PI3K (Tyr458), Akt or phospho-Akt (Ser473), or eNOS or phospho-eNOS (Ser1177). Identical results were obtained in three separate rat hearts at each time point. Normalization of Western blot was ensured by GAPDH.
