Wen-pi-tang-Hab-Wu-ling-san, a Polyherbal Medicine, Attenuates ER Stress in 3T3-L1 Preadipocytes by Promoting the Insulin Signaling Pathway
Figure 1
Effect of WHW extract on tunicamycin-induced ER stress indicators in 3T3-L1 cells. The cells were treated with WHW extract at concentrations of 500 and 1000 μg/mL for 9 days. (a) The expressions of XBP-1, GRP 78, and GAPDH mRNA were analyzed by RT-PCR. GAPDH was used as the internal control. (b) The expression of eIF2α, CHOP10, or GRP78 was measured by western blot analysis. Relative density was calculated as the ratio of p-eIF2α expression to eIF2α expression or of actin expression to expressions of CHOP10 or GRP78 expression, respectively. Con: differentiated adipocytes as a control; Tu: 2 μg/mL tunicamycin; 500: 2 μg/mL tunicamycin + WHW 500 μg/mL; 1000: 2 μg/mL tunicamycin + WHW 1000 μg/mL. These data are presented as the mean ± SD (). *Significantly different () from tunicamycin treatment.
