Subamolide A Induces Mitotic Catastrophe Accompanied by Apoptosis in Human Lung Cancer Cells
Figure 1
The effects of Sub-A on cell proliferation and colony formation in lung cancer cell lines A549 and NCI-H460. (a) The chemical structure of Sub-A isolated from the leaves of Cinnamomum subavenium Miq. (b) Decreased A549 and NCI-H460 cell viability by Sub-A. (c) The influence of A549 and NCI-H460 on the number of colony-forming cells, as evaluated by clonogenic assay. (d) Sub-A did not affect the cell viability in IMR-90 cells. The cell viability was assessed by trypan blue staining after 48 h treatment at difference of concentration of Sub-A. For colony-forming assay, the clonogenic assay was performed as described in Section 2. Results were expressed as the percentage of cell proliferation relative to the proliferation of the control. The data shown are the mean from three independent experiments. Each value in the data was the mean ± SD of three determinations.
