Subamolide A Induces Mitotic Catastrophe Accompanied by Apoptosis in Human Lung Cancer Cells
Figure 8
Sub-A inhibited growth of A549 in nude mice. (a) The mean of tumor volume measured at the indicated number of days after implantation. (b) There were no signs of toxicity, as judged by parallel monitoring of body weight and tissue sections of lungs, livers, and kidneys, in Sub-A-treated mice. Sub-A induced (c) mitotic catastrophe and (d) apoptotic cell death in the A549 xenograft. Animals bearing preestablished tumors ( per group) were dosed daily for 53 days with IP injections of low-dose Sub-A (4 mg/kg/d), high-dose sub-A (10 mg/kg/d), or vehicle. During the 53-day treatment, tumor volumes were estimated using measurements taken by external calipers (mm3). The induction of mitotic catastrophe was assessed by TEM and apoptosis was determined by TUNEL assay. The asterisk indicated a significant difference between Sub-A-treated cells and the controls (, by ANOVA with Student’s -test post hoc).
