Freshwater Clam Extract Ameliorates Triglyceride and Cholesterol Metabolism through the Expression of Genes Involved in Hepatic Lipogenesis and Cholesterol Degradation in Rats
Figure 2
Hepatic levels of the genes involved in cholesterol metabolism, bile acid biosynthesis, and transport and biosynthesis of fatty acids in rats fed FCE and SPI. In the first experiment, the levels of mRNA for CYP7A1, ABCG5, FAS, SCD1, and ELOVL6 were measured by northern blotting. Each value is expressed as the mean ± SEM for 6 rats in each group (control (C), FCE (F), SPI (S)). The statistical significance of differences among values was analyzed by ANOVA and then by Tukey’s multiple-range test. Values with different letters indicate a statistically significant difference, (a). In the second experiment (control group (C) versus FCE group (F)), the levels of mRNA for ACACA, FABP2, FABP5, apo A-I, and SREBP-1 were measured by northern blotting. Each value is expressed as the mean ± SEM for 6 rats in each group. The statistical significance of differences among values was analyzed by Student’s -test. In each graph, * and *** indicate statistically significant difference at and , respectively (b). ApoE was used as a normalization standard, as its levels were not significantly changed in our experiments. FCE, freshwater clam extract; SPI, soy protein extract; CYP7A1, cytochrome P-450 7A1; FAS, fatty acid synthase; ACACA, acetyl-coenzyme A carboxylase alpha; SCD1, stearoyl-coenzyme A desaturase 1; FABP2, fatty acid-binding protein 2 intestinal; FABP5, fatty acid-binding protein 5 epidermal; SREBP-1, sterol regulatory element-binding protein 1; ABCG5, ATP-binding cassette subfamily G, member 5; ELOVL6, elongation of very long-chain fatty acids protein 6; Apo A-I, apolipoprotein A-1.
