Bisdemethoxycurcumin Increases Sirt1 to Antagonize t-BHP-Induced Premature Senescence in WI38 Fibroblast Cells
Figure 4
BDMC activated AMPK with the increase in Sirt1. (a) WI38 cells were treated with different doses of BDMC (5–40 μM) for 48 h. Then, phosphorylation level of AMPK and total AMPK protein level were measured by Western blot assay. (b) WI38 cells were treated with nicotinamide (NAM) for 1-2 h, followed by BDMC (20 μM) for 48 h; then Sirt1, p-AMPK, and AMPK were examined by Western blot assay.
