Water Extract of Dryopteris crassirhizoma Attenuates Bone Loss by Suppressing Osteoclast Differentiation and Function
Figure 3
WEDC abrogates RANKL-induced c-Fos and NFATc1 expression in BMMs. BMMs were pretreated with or without WEDC (50 μg/mL) for 3 h and cultured in the presence of M-CSF (60 ng/mL) and RANKL (100 ng/mL) for 4 days. Total RNA and cell lysates were isolated at the indicated time points. (a) mRNA levels of c-Fos and NFATc1 were analyzed by QPCR. Data are expressed as mean ± SD and are representative of three experiments. ** versus vehicle-treated control group. (b) Total cell lysates were analyzed by Western blot analysis with the indicated antibodies. GAPDH was used as a loading control.
