Water Extract of Dryopteris crassirhizoma Attenuates Bone Loss by Suppressing Osteoclast Differentiation and Function
Figure 5
WEDC decreases bone-resorbing activity of mature osteoclasts. Mature osteoclasts were cultured with vehicle or WEDC on an Osteo Assay Surface plate for 24 h. (a) Representative microscopic pictures of TRAP staining (upper panel) and resorbed pits (down panel). (b) The number of TRAP(+)MNCs having more than three nuclei. (c) The total areas of resorbed pits. Data are expressed as mean ± SD and are representative of three experiments. * and ** versus vehicle-treated control. (d) Mature osteoclasts were treated with vehicle or WEDC for 30 min, and F-actin was visualized with fluorescence microscopy.
