Use of Propolis Hydroalcoholic Extract to Treat Colitis Experimentally Induced in Rats by 2,4,6-Trinitrobenzenesulfonic Acid
Figure 2
Photomicrography of the distal colons of rats that received rectal TNBS (to induce colitis) or saline solution (S) after treatment for 5 or 12 days with propolis hydroalcoholic solution (TNBS-P and SP) or mesalazine solution (TNBS-M). The animals were euthanized 7 or 14 days after the colitis was induced. The animals in groups S ((a) and (b)) and SP ((e) and (f)) exhibited normal histological characteristics (m: mucosa, sm: submucosa, and mu: muscular). Group TNBS ((c) and (d)). (c) shows extensive ulceration and intense inflammatory infiltrate surrounded by normal mucosa; a crypt abscess containing polymorphonuclear cells is shown in detail. In (d), note the distorted crypts (c), presence of foreign body giant cells in the submucosa (detail), and expansion of the serosa (s). The group TNBS-P ((g) and (h)) mucosa shows regeneration with bifurcated crypts (c) and cysts (*) in (g) and serosa expansion (S) in (h). Group TNBS-M ((i) and (j)). In (i), note the ulcerated area in the mucosa and intense transmural inflammatory infiltrate. In the detail, note the presence of eosinophils inside a myenteric ganglion. In (j), note the restored mucosa, absence of inflammatory infiltrate, and expanded serosa (s). Hematoxylin-eosin. Scale = 200 μm.
