Induction of Apoptosis and Cell Cycle Blockade by Helichrysetin in A549 Human Lung Adenocarcinoma Cells
Figure 4
(a) TUNEL assay detects DNA fragmentation in cells. Results showed that the percentage of TUNEL-positive cells increased significantly in a dose- and time-dependent manner, presented as mean ± S.E., and values of were regarded as statistically significant compared to the untreated control. (b) A549 cells were treated with helichrysetin at 5 μg/mL, 15 μg/mL, and 20 μg/mL (top) and 24 h, 48 h, and 72 h (bottom). Density plots show the increase of Alexa Fluor fluorescence intensity as the dose and time increases.
